Overview
Product name
Anti-mouse IgG for IP (HRP)See all IgG secondary antibodiesHost species
RatTarget species
MouseTested applications
Suitable for:WBmore detailsConjugation
HRP
Properties
Form
LiquidStorage instructions
Shipped at 4°C. Store at +4°C.Storage buffer
pH: 7.20Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 50% Glycerol, 0.1% BSA
Concentration information loading...Clonality
MonoclonalIsotype
IgGGeneral notes
Number of blots: At least 20 blots, based on a 1 µl/ml dilutionand 5 ml diluted antibody per blot. Important protocol notes: 1. Theanti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.3. IP sample should be completely reduced/denatured before loaded onto a western blot.4. Milk should be used as the blocking protein for the immunoblot.Western blot and IP resources: a) Western blot a beginner"s guide b) IP protocol c) IP troubleshooting tips
Research areas
- Immunology
- Immunoglobulins
- Heavy Chain
- IgG
- Secondary antibodies
- anti-Mouse
- IgG
- Enzyme
- HRP
- Isotype/Loading Controls
- Isotype Controls
- Mouse
Associated products
Related Products
- Anti-mouse IgG for IP (Biotin) (ab131367)
- Goat Anti-Mouse IgG H&L (HRP) (ab97023)
- Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)
- Rat monoclonal [JC5-1] Anti-Mouse lambda light chain (HRP) (ab99625)
Applications
Our Abpromise guarantee covers the use ofab131368in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. The optimal dilution will depend on the sensitivity of the HRP substrate. |
Images
Western blot - Anti-mouse IgG for IP (HRP) (ab131368)IP sample preparation: Caspase 7 was immunoprecipitated from 0.5 ml of 1x107 Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5µg mouse anti-human Caspase 7.
WB conditions: Precipitate from 1x106 cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.
Detection: Lane 1: Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368) Lane 2: Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibodyLane 3: Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.
Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.
Protocols
- Protocol Booklet
Click here to view the general protocols
Datasheets and documents
- Datasheet
- SDS
References
ab131368 has been referenced in 82 publications.
- Luo Z et al. Silencing of RBP-JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Mol Med Rep 21:69-76 (2020). PubMed: 31746399
- Zhang Z et al. LncRNA DRAIC inhibits proliferation and metastasis of gastric cancer cells through interfering with NFRKB deubiquitination mediated by UCHL5. Cell Mol Biol Lett 25:29 (2020). PubMed: 32351584
- Kouloulia S et al. Raptor-Mediated Proteasomal Degradation of Deamidated 4E-BP2 Regulates Postnatal Neuronal Translation and NF-?B Activity. Cell Rep 29:3620-3635.e7 (2019). PubMed: 31825840
- Anczurowski M et al. Chaperones of the class I peptide-loading complex facilitate the constitutive presentation of endogenous antigens on HLA-DP84GGPM87. J Autoimmun 102:114-125 (2019). PubMed: 31078377
- Wei X et al. miR-21 inhibitor facilitates the anticancer activity of doxorubicin loaded nanometer in melanoma. Oncol Rep 42:414-424 (2019). PubMed: 31115580
